Antiadhesive character of mucin O-glycans at the apical surface of corneal epithelial cells.

ABSTRACT:

Purpose

Prolonged contact of opposite mucosal surfaces, which occurs on the ocular surface, oral cavity, reproductive tract, and gut, requires a specialized apical cell surface that prevents adhesion. The purpose of this study was to evaluate the contribution of mucin O-glycans to the antiadhesive character of human corneal-limbal epithelial (HCLE) cells.

Methods

Mucin O-glycan biosynthesis in HCLE cells was disrupted by metabolic interference with benzyl-alpha-GalNAc. The cell surface mucin MUC16 and its carbohydrate epitope H185 were detected by immunofluorescence and Western blot. HCLE cell surface features were assessed by field emission scanning electron microscopy. Cell-cell adhesion assays were performed under static conditions and in a parallel plate laminar flow cha

SUBMITTER: Sumiyoshi M 

PROVIDER: S-EPMC2247472 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature