Temporal transcriptomic analysis of the Listeria monocytogenes EGD-e sigmaB regulon.

ABSTRACT:

Background

The opportunistic food-borne gram-positive pathogen Listeria monocytogenes can exist as a free-living microorganism in the environment and grow in the cytoplasm of vertebrate and invertebrate cells following infection. The general stress response, controlled by the alternative sigma factor, sigmaB, has an important role for bacterial survival both in the environment and during infection. We used quantitative real-time PCR analysis and immuno-blot analysis to examine sigmaB expression during growth of L. monocytogenes EGD-e. Whole genome-based transcriptional profiling was used to identify sigmaB-dependent genes at different growth phases.

Results

We detected 105 sigmaB-positively regulated genes and 111 genes which appeared to be under negative control of sigmaB a

SUBMITTER: Hain T 

PROVIDER: S-EPMC2248587 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature