Genome wide screens in yeast to identify potential binding sites and target genes of DNA-binding proteins.

ABSTRACT: Knowledge of all binding sites for transcriptional activators and repressors is essential for computationally aided identification of transcriptional networks. The techniques developed for defining the binding sites of transcription factors tend to be cumbersome and not adaptable to high throughput. We refined a versatile yeast strategy to rapidly and efficiently identify genomic targets of DNA-binding proteins. Yeast expressing a transcription factor is mated to yeast containing a library of genomic fragments cloned upstream of the reporter gene URA3. DNA fragments with target-binding sites are identified by growth of yeast clones in media lacking uracil. The experimental approach was validated with the tumor suppressor protein p53 and the forkhead protein FoxI1 using genomic libraries fo

SUBMITTER: Zeng J 

PROVIDER: S-EPMC2248728 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature