Investigation of protein refolding using a fractional factorial screen: a study of reagent effects and interactions.

ABSTRACT: A recurring obstacle for structural genomics is the expression of insoluble, aggregated proteins. In these cases, the use of alternative salvage strategies, like in vitro refolding, is hindered by the lack of a universal refolding method. To overcome this obstacle, fractional factorial screens have been introduced as a systematic and rapid method to identify refolding conditions. However, methodical analyses of the effectiveness of refolding reagents on large sets of proteins remain limited. In this study, we address this void by designing a fractional factorial screen to rapidly explore the effect of 14 different reagents on the refolding of 33 structurally and functionally diverse proteins. The refolding data was analyzed using statistical methods to determine the effect of each refoldin

SUBMITTER: Willis MS 

PROVIDER: S-EPMC2253366 | biostudies-literature | 2005 Jul

REPOSITORIES: biostudies-literature