Acetylation of histone H4K4 is cell cycle regulated and mediated by HAT3 in Trypanosoma brucei.

ABSTRACT: Post-translational histone modifications have been studied intensively in several eukaryotes. It has been proposed that these modifications constitute a 'histone code' that specifies epigenetic information for transcription regulation. With a limited number of histone-modifying enzymes, implying less redundancy, Trypanosoma brucei represents an excellent system in which to investigate the function of individual histone modifications and histone-modifying enzymes. In this study, we characterized the acetylation of lysine 4 of histone H4 (H4K4), the most abundant acetylation site in T. brucei histones. Because of the large sequence divergence of T. brucei histones, we generated highly specific antibodies to acetylated and unmodified H4K4. Immunofluorescence microscopy and Western blots with

SUBMITTER: Siegel TN 

PROVIDER: S-EPMC2253726 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature