A natural antisense transcript of the Petunia hybrida Sho gene suggests a role for an antisense mechanism in cytokinin regulation.

ABSTRACT: The Sho gene from Petunia hybrida encodes an enzyme responsible for the synthesis of plant cytokinins. The 3' region of the Sho gene contains a promoter in the opposite orientation that produces a partially overlapping antisense transcript. Although Sho expression varies significantly in individual cell types, the sense and antisense transcript levels maintain a stable ratio in most tissue types. In reporter lines for the antisense promoter, we observed a change in antisense promoter activity in newly formed tissue that had been induced by prolonged culture on cytokinins or following decapitation. We interpret these data as a reflection of tissue-specific threshold levels for activation of the antisense transcript. In all tissue types tested, we detect a pool of antisense RNA of approximat

SUBMITTER: Zubko E 

PROVIDER: S-EPMC2253869 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature