A direct repeat of E-box-like elements is required for cell-autonomous circadian rhythm of clock genes.

ABSTRACT:

Background

The circadian expression of the mammalian clock genes is based on transcriptional feedback loops. Two basic helix-loop-helix (bHLH) PAS (for Period-Arnt-Sim) domain-containing transcriptional activators, CLOCK and BMAL1, are known to regulate gene expression by interacting with a promoter element termed the E-box (CACGTG). The non-canonical E-boxes or E-box-like sequences have also been reported to be necessary for circadian oscillation.

Results

We report a new cis-element required for cell-autonomous circadian transcription of clock genes. This new element consists of a canonical E-box or a non-canonical E-box and an E-box-like sequence in tandem with the latter with a short interval, 6 base pairs, between them. We demonstrate that both E-box or E-box-like sequen

SUBMITTER: Nakahata Y 

PROVIDER: S-EPMC2254435 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature