Amplification biases: possible differences among deviating gene expressions.

ABSTRACT:

Background

Gene expression profiling has become a tool of choice to study pathological or developmental questions but in most cases the material is scarce and requires sample amplification. Two main procedures have been used: in vitro transcription (IVT) and polymerase chain reaction (PCR), the former known as linear and the latter as exponential. Previous reports identified enzymatic pitfalls in PCR and IVT protocols; however the possible differences between the sequences affected by these amplification defaults were only rarely explored.

Results

Screening a bovine cDNA array dedicated to embryonic stages with embryonic (n = 3) and somatic tissues (n = 2), we proceeded to moderate amplifications starting from 1 mug of total RNA (global PCR or IVT one round). Whatever the ti

SUBMITTER: Degrelle SA 

PROVIDER: S-EPMC2257942 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature