Strategy for in situ detection of natural transformation-based horizontal gene transfer events.

ABSTRACT: A strategy is described that enables the in situ detection of natural transformation in Acinetobacter baylyi BD413 by the expression of a green fluorescent protein. Microscale detection of bacterial transformants growing on plant tissues was shown by fluorescence microscopy and indicated that cultivation-based selection of transformants on antibiotic-containing agar plates underestimates transformation frequencies.

SUBMITTER: Rizzi A 

PROVIDER: S-EPMC2258602 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature