Lipase expression in Pseudomonas alcaligenes is under the control of a two-component regulatory system.

ABSTRACT: Preliminary observations in a large-scale fermentation process suggested that the lipase expression of Pseudomonas alcaligenes can be switched on by the addition of certain medium components, such as soybean oil. In an attempt to elucidate the mechanism of induction of lipase expression, we have set up a search method for genes controlling lipase expression by use of a cosmid library containing fragments of P. alcaligenes genomic DNA. A screen for lipase hyperproduction resulted in the selection of multiple transformants, of which the best-producing strains comprised cosmids that shared an overlapping genomic fragment. Within this fragment, two previously unidentified genes were found and named lipQ and lipR. Their encoded proteins belong to the NtrBC family of regulators that regulate gen

SUBMITTER: Krzeslak J 

PROVIDER: S-EPMC2258621 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature