Rapid DNA library construction for functional genomic and metagenomic screening.

ABSTRACT: A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins were cloned from bacteriophage genomes and an aerolysin was cloned from a metagenomic sample.

SUBMITTER: Schmitz JE 

PROVIDER: S-EPMC2258639 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature