Genetic analysis of lipooligosaccharide core biosynthesis in Campylobacter jejuni 81-176.

ABSTRACT: We report isolation and characterization of Campylobacter jejuni 81-176 lgtF and galT lipooligosaccharide (LOS) core mutants. It has been suggested that the lgtF gene of C. jejuni encodes a two-domain glucosyltransferase that is responsible for the transfer of a beta-1,4-glucose residue on heptosyltransferase I (Hep I) and for the transfer of a beta-1,2-glucose residue on Hep II. A site-specific mutation in the lgtF gene of C. jejuni 81-176 resulted in expression of a truncated LOS, and complementation of the mutant in trans restored the core mobility to that of the wild type. Mass spectrometry and nuclear magnetic resonance of the truncated LOS confirmed the loss of two glucose residues, a beta-1,4-glucose on Hep I and a beta-1,2-glucose on Hep II. Mutation of another gene, galT, encoding

SUBMITTER: Kanipes MI 

PROVIDER: S-EPMC2258656 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature