CmeR functions as a pleiotropic regulator and is required for optimal colonization of Campylobacter jejuni in vivo.

ABSTRACT: CmeR functions as a transcriptional repressor modulating the expression of the multidrug efflux pump CmeABC in Campylobacter jejuni. To determine if CmeR also regulates other genes in C. jejuni, we compared the transcriptome of the cmeR mutant with that of the wild-type strain using a DNA microarray. This comparison identified 28 genes that showed a > or = 2-fold change in expression in the cmeR mutant. Independent real-time quantitative reverse transcription-PCR experiments confirmed 27 of the 28 differentially expressed genes. The CmeR-regulated genes encode membrane transporters, proteins involved in C4-dicarboxylate transport and utilization, enzymes for biosynthesis of capsular polysaccharide, and hypothetical proteins with unknown functions. Among the genes whose expression was upreg

SUBMITTER: Guo B 

PROVIDER: S-EPMC2258875 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature