Structure of antibody-neutralized murine norovirus and unexpected differences from viruslike particles.

ABSTRACT: Noroviruses (family Caliciviridae) are the major cause of epidemic nonbacterial gastroenteritis in humans, but the mechanism of antibody neutralization is unknown and no structure of an infectious virion has been reported. Murine norovirus (MNV) is the only norovirus that can be grown in tissue culture, studied in an animal model, and reverse engineered via an infectious clone and to which neutralizing antibodies have been isolated. Presented here are the cryoelectron microscopy structures of an MNV virion and the virion in complex with neutralizing Fab fragments. The most striking differences between MNV and previous calicivirus structures are that the protruding domain is lifted off the shell domain by approximately 16A and rotated approximately 40 degrees in a clockwise fashion and form

SUBMITTER: Katpally U 

PROVIDER: S-EPMC2258928 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature