Probing regulon of ArcA in Shewanella oneidensis MR-1 by integrated genomic analyses.

ABSTRACT:

Background

The Arc two-component system is a global regulator controlling many genes involved in aerobic/anaerobic respiration and fermentative metabolism in Escherichia coli. Shewanella oneidensis MR-1 contains a gene encoding a putative ArcA homolog with ~81% amino acid sequence identity to the E. coli ArcA protein but not a full-length arcB gene.

Results

To understand the role of ArcA in S. oneidensis, an arcA deletion strain was constructed and subjected to both physiological characterization and microarray analysis. Compared to the wild-type MR-1, the mutant exhibited impaired aerobic growth and a defect in utilizing DMSO in the absence of O2. Microarray analyses on cells grown aerobically and anaerobically on fumarate revealed that expression of 1009 genes was signific

SUBMITTER: Gao H 

PROVIDER: S-EPMC2262068 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature