Construction and characterization of an expressed sequenced tag library for the mosquito vector Armigeres subalbatus.

ABSTRACT:

Background

The mosquito, Armigeres subalbatus, mounts a distinctively robust innate immune response when infected with the nematode Brugia malayi, a causative agent of lymphatic filariasis. In order to mine the transcriptome for new insight into the cascade of events that takes place in response to infection in this mosquito, 6 cDNA libraries were generated from tissues of adult female mosquitoes subjected to immune-response activation treatments that lead to well-characterized responses, and from aging, naïve mosquitoes. Expressed sequence tags (ESTs) from each library were produced, annotated, and subjected to comparative analyses.

Results

Six libraries were constructed and used to generate 44,940 expressed sequence tags, of which 38,079 passed quality filters to be includ

SUBMITTER: Mayhew GF 

PROVIDER: S-EPMC2262096 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature