Transcriptomic analyses reveal novel genes with sexually dimorphic expression in the zebrafish gonad and brain.

ABSTRACT:

Background

Our knowledge on zebrafish reproduction is very limited. We generated a gonad-derived cDNA microarray from zebrafish and used it to analyze large-scale gene expression profiles in adult gonads and other organs.

Methodology/principal findings

We have identified 116638 gonad-derived zebrafish expressed sequence tags (ESTs), 21% of which were isolated in our lab. Following in silico normalization, we constructed a gonad-derived microarray comprising 6370 unique, full-length cDNAs from differentiating and adult gonads. Labeled targets from adult gonad, brain, kidney and 'rest-of-body' from both sexes were hybridized onto the microarray. Our analyses revealed 1366, 881 and 656 differentially expressed transcripts (34.7% novel) that showed highest expression in ovary, t

SUBMITTER: Sreenivasan R 

PROVIDER: S-EPMC2262149 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature