The efficacy of DNA vaccination is enhanced in mice by targeting the encoded protein to dendritic cells.

ABSTRACT: DNA vaccines promote an immune response by providing antigen-encoding DNA to the recipient, but the efficacy of such vaccines needs improving. Many approaches have considerable potential but currently induce relatively weak immune responses despite multiple high doses of DNA vaccine. Here, we asked whether targeting vaccine antigens to DCs would increase the immunity and protection that result from DNA vaccines. To determine this, we generated a DNA vaccine encoding a fusion protein comprised of the vaccine antigen and a single-chain Fv antibody (scFv) specific for the DC-restricted antigen-uptake receptor DEC205. Following vaccination of mice, the vaccine antigen was expressed selectively by DCs, which were required for the increased efficacy of MHC class I and MHC class II antigen presen

SUBMITTER: Nchinda G 

PROVIDER: S-EPMC2263146 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature