Nanometer-scale mapping and single-molecule detection with color-coded nanoparticle probes.

ABSTRACT: We report a method for single-molecule detection and biomolecular structural mapping based on dual-color imaging and automated colocalization of bioconjugated nanoparticle probes at nanometer precision. In comparison with organic dyes and fluorescent proteins, nanoparticle probes such as fluorescence energy-transfer nanobeads and quantum dots provide significant advantages in signal brightness, photostability, and multicolor-light emission. As a result, we have achieved routine two-color superresolution imaging and single-molecule detection with standard fluorescence microscopes and inexpensive digital color cameras. By using green and red nanoparticles to simultaneously recognize two binding sites on a single target, individual biomolecules such as nucleic acids are detected and identifie

SUBMITTER: Agrawal A 

PROVIDER: S-EPMC2265145 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature