Multiple independent origins of a protease inhibitor resistance mutation in salvage therapy patients.

ABSTRACT:

Background

Combination anti-viral therapies have reduced treatment failure rates by requiring multiple specific mutations to be selected on the same viral genome to impart high-level drug resistance. To determine if the common protease inhibitor resistance mutation L90M is only selected once or repeatedly on different HIV genetic backbones during the course of failed anti-viral therapies we analyzed a linked region of the viral genome during the evolution of multi-drug resistance.

Results

Using L90M allele specific PCR we amplified and sequenced gag-pro regions linked to very early L90M containing HIV variants prior to their emergence and detection as dominant viruses in 15 failed salvage therapy patients. The early minority L90M linked sequences were then compared to those

SUBMITTER: Kapoor A 

PROVIDER: S-EPMC2265302 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature