Differential functions for the transcription factor E2A in positive and negative gene regulation in pre-B lymphocytes.

ABSTRACT: The transcription factors encoded by the E2A gene have been shown to play essential roles in the initiation and progression of lymphocyte development. However, there is still a lack of comprehensive understanding of E2A downstream genes in B-cell development. We previously developed a gene tagging-based chromatin immunoprecipitation (ChIP) system to directly evaluate E2A target genes in B-cell development. Here, we have improved this ChIP strategy and used it in conjunction with microarray analysis on E2A-deficient pre-B-cell lines to determine E2A target genes in lymphocyte development. Both microarray data and ChIP studies confirmed that E2A directly controls IgH gene expression. The microarray assay also revealed genes that were significantly up-regulated after E2A disruption. ChIP anal

SUBMITTER: Greenbaum S 

PROVIDER: S-EPMC2265379 | biostudies-literature | 2004 Oct

REPOSITORIES: biostudies-literature