Transcription and chromatin organization of a housekeeping gene cluster containing an integrated beta-globin locus control region.

ABSTRACT: The activity of locus control regions (LCR) has been correlated with chromatin decondensation, spreading of active chromatin marks, locus repositioning away from its chromosome territory (CT), increased association with transcription factories, and long-range interactions via chromatin looping. To investigate the relative importance of these events in the regulation of gene expression, we targeted the human beta-globin LCR in two opposite orientations to a gene-dense region in the mouse genome containing mostly housekeeping genes. We found that each oppositely oriented LCR influenced gene expression on both sides of the integration site and over a maximum distance of 150 kilobases. A subset of genes was transcriptionally enhanced, some of which in an LCR orientation-dependent manner. The l

SUBMITTER: Noordermeer D 

PROVIDER: S-EPMC2265466 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature