Uncovering a macrophage transcriptional program by integrating evidence from motif scanning and expression dynamics.

ABSTRACT: Macrophages are versatile immune cells that can detect a variety of pathogen-associated molecular patterns through their Toll-like receptors (TLRs). In response to microbial challenge, the TLR-stimulated macrophage undergoes an activation program controlled by a dynamically inducible transcriptional regulatory network. Mapping a complex mammalian transcriptional network poses significant challenges and requires the integration of multiple experimental data types. In this work, we inferred a transcriptional network underlying TLR-stimulated murine macrophage activation. Microarray-based expression profiling and transcription factor binding site motif scanning were used to infer a network of associations between transcription factor genes and clusters of co-expressed target genes. The time-l

SUBMITTER: Ramsey SA 

PROVIDER: S-EPMC2265556 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature