Checkpoint effects and telomere amplification during DNA re-replication in fission yeast.

ABSTRACT:

Background

Although much is known about molecular mechanisms that prevent re-initiation of DNA replication on newly replicated DNA during a single cell cycle, knowledge is sparse regarding the regions that are most susceptible to re-replication when those mechanisms are bypassed and regarding the extents to which checkpoint pathways modulate re-replication. We used microarrays to learn more about these issues in wild-type and checkpoint-mutant cells of the fission yeast, Schizosaccharomyces pombe.

Results

We found that over-expressing a non-phosphorylatable form of the replication-initiation protein, Cdc18 (known as Cdc6 in other eukaryotes), drove re-replication of DNA sequences genome-wide, rather than forcing high level amplification of just a few sequences. Moderate vari

SUBMITTER: Mickle KL 

PROVIDER: S-EPMC2265721 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature