Spherezymes: a novel structured self-immobilisation enzyme technology.

ABSTRACT:

Background

Enzymes have found extensive and growing application in the field of chemical organic synthesis and resolution of chiral intermediates. In order to stabilise the enzymes and to facilitate their recovery and recycle, they are frequently immobilised. However, immobilisation onto solid supports greatly reduces the volumetric and specific activity of the biocatalysts. An alternative is to form self-immobilised enzyme particles.

Results

Through addition of protein cross-linking agents to a water-in-oil emulsion of an aqueous enzyme solution, structured self-immobilised spherical enzyme particles of Pseudomonas fluorescens lipase were formed. The particles could be recovered from the emulsion, and activity in aqueous and organic solvents was successfully demonstrated. P

SUBMITTER: Brady D 

PROVIDER: S-EPMC2266724 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature