Demonstration of IS711 transposition in Brucella ovis and Brucella pinnipedialis.

ABSTRACT:

Background

The Brucella genome contains an insertion sequence (IS) element called IS711 or IS6501, which is specific to the genus. The copy number of IS711 varies in the genome of the different Brucella species, ranging from 7 in B. abortus, B. melitensis and B. suis to more than 30 in B. ovis and in Brucella strains isolated from marine mammals. At present, there is no experimental evidence of transposition of IS711, but the occurrence of this element with a high copy number in some species, and the isolation of Brucella strains with "ectopic" copies of IS711 suggested that this IS could still transpose.

Results

In this study we obtained evidence of transposition of IS711 from the B. ovis and B. pinnipedialis chromosomes by using the "transposon trap" plasmid pGBG1. This pl

SUBMITTER: Ocampo-Sosa AA 

PROVIDER: S-EPMC2266754 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature