Gene expression of purified beta-cell tissue obtained from human pancreas with laser capture microdissection.

ABSTRACT:

Context

Human beta-cell gene profiling is a powerful tool for understanding beta-cell biology in normal and pathological conditions. Assessment is complicated when isolated islets are studied because of contamination by non-beta-cells and the trauma of the isolation procedure.

Objective

The objective was to use laser capture microdissection (LCM) of human beta-cells from pancreases of cadaver donors and compare their gene expression with that of handpicked isolated islets.

Design

Endogenous autofluorescence of beta-cells facilitated procurement of purified beta-cell tissue from frozen pancreatic sections with LCM. Gene expression profiles of three microdissected beta-cell samples and three isolated islet preparations were obtained. The array data were normalized using

SUBMITTER: Marselli L 

PROVIDER: S-EPMC2266961 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature