A dual-color fluorescence-based platform to identify selective inhibitors of Akt signaling.

ABSTRACT:

Background

Inhibition of Akt signaling is considered one of the most promising therapeutic strategies for many cancers. However, rational target-orientated approaches to cell based drug screens for anti-cancer agents have historically been compromised by the notorious absence of suitable control cells.

Methodology/principal findings

In order to address this fundamental problem, we have developed BaFiso, a live-cell screening platform to identify specific inhibitors of this pathway. BaFiso relies on the co-culture of isogenic cell lines that have been engineered to sustain interleukin-3 independent survival of the parental Ba/F3 cells, and that are individually tagged with different fluorescent proteins. Whilst in the first of these two lines cell survival in the absence of I

SUBMITTER: Rosado A 

PROVIDER: S-EPMC2267216 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature