A highly sensitive and specific system for large-scale gene expression profiling.

ABSTRACT:

Background

Rapid progress in the field of gene expression-based molecular network integration has generated strong demand on enhancing the sensitivity and data accuracy of experimental systems. To meet the need, a high-throughput gene profiling system of high specificity and sensitivity has been developed.

Results

By using specially designed primers, the new system amplifies sequences in neighboring exons separated by big introns so that mRNA sequences may be effectively discriminated from other highly related sequences including their genes, unprocessed transcripts, pseudogenes and pseudogene transcripts. Probes used for microarray detection consist of sequences in the two neighboring exons amplified by the primers. In conjunction with a newly developed high-throughput mult

SUBMITTER: Hu G 

PROVIDER: S-EPMC2267712 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature