Dynamic remodeling of individual nucleosomes across a eukaryotic genome in response to transcriptional perturbation.

ABSTRACT: The eukaryotic genome is packaged as chromatin with nucleosomes comprising its basic structural unit, but the detailed structure of chromatin and its dynamic remodeling in terms of individual nucleosome positions has not been completely defined experimentally for any genome. We used ultra-high-throughput sequencing to map the remodeling of individual nucleosomes throughout the yeast genome before and after a physiological perturbation that causes genome-wide transcriptional changes. Nearly 80% of the genome is covered by positioned nucleosomes occurring in a limited number of stereotypical patterns in relation to transcribed regions and transcription factor binding sites. Chromatin remodeling in response to physiological perturbation was typically associated with the eviction, appearance,

SUBMITTER: Shivaswamy S 

PROVIDER: S-EPMC2267817 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature