Translation of cIAP2 mRNA is mediated exclusively by a stress-modulated ribosome shunt.

ABSTRACT: During cellular stress, translation persists or increases for a number of stress-responsive proteins, including cellular inhibitor of apoptosis 2 (cIAP2). The cIAP2 transcript includes a very long (2.78-kb) 5' untranslated region (UTR) with an unusually high number of upstream AUGs (uAUGs), i.e., 64, and a stable predicted secondary structure (DeltaG congruent with -620 kcal/mol) that should completely block conventional scanning-dependent translation initiation. This region did not facilitate internal ribosome entry in vitro or when RNA reporter transcripts were transfected into cells. However, several structural features within the cIAP2 5' UTR were observed to be nearly identical to those required for ribosome shunting in cauliflower mosaic virus RNA and are well conserved in cIAP2 orth

SUBMITTER: Sherrill KW 

PROVIDER: S-EPMC2268400 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature