Aberrant epigenetic changes and gene expression in cloned cattle dying around birth.

ABSTRACT:

Background

Aberrant reprogramming of donor somatic cell nuclei may result in many severe problems in animal cloning. To assess the extent of abnormal epigenetic modifications and gene expression in clones, we simultaneously examined DNA methylation, histone H4 acetylation and expression of six genes (beta-actin, VEGF, oct4, TERT, H19 and Igf2) and a repetitive sequence (art2) in five organs (heart, liver, spleen, lung and kidney) from two cloned cattle groups that had died at different stages. In the ED group (early death, n = 3), the cloned cattle died in the perinatal period. The cattle in the LD group (late death, n = 3) died after the perinatal period. Normally reproduced cattle served as a control group (n = 3).

Results

Aberrant DNA methylation, histone H4 acetylation a

SUBMITTER: Lin L 

PROVIDER: S-EPMC2268668 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature