Rapid acid treatment of Escherichia coli: transcriptomic response and recovery.

ABSTRACT:

Background

Many E. coli genes show pH-dependent expression during logarithmic growth in acid (pH 5-6) or in base (pH 8-9). The effect of rapid pH change, however, has rarely been tested. Rapid acid treatment could distinguish between genes responding to external pH, and genes responding to cytoplasmic acidification, which occurs transiently following rapid external acidification. It could reveal previously unknown acid-stress genes whose effects are transient, as well as show which acid-stress genes have a delayed response.

Results

Microarray hybridization was employed to observe the global gene expression of E. coli K-12 W3110 following rapid acidification of the external medium, from pH 7.6 to pH 5.5. Fluorimetric observation of pH-dependent tetR-YFP showed that rapid exte

SUBMITTER: Kannan G 

PROVIDER: S-EPMC2270276 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature