The biodegradability of electrospun Dextran/PLGA scaffold in a fibroblast/macrophage co-culture.

ABSTRACT: Fibroblast and macrophage are 2 dominant cell types respond cooperatively to degrade implanted biomaterials. Using an electrospun Dextran/Poly-lactide-co-glycolide (PLGA) scaffold as a model, an in vitro fibroblast/macrophage co-culture system was developed to investigate the degradability of implantable biodegradable materials. SEM showed that both fibroblasts and macrophages were able to degrade the scaffold, separately or cooperatively. Under the synergistic coordination of macrophages and fibroblasts, scaffolds showed faster degradation rate than their counterparts incubated with a single type of cells as well as in PBS or cell culture medium. Lysozyme, non-specific esterase (NSE), gelatinase, hyaluronidase-1 and alpha-glucosidase were up-regulated in the presence of the scaffold, sugg

SUBMITTER: Pan H 

PROVIDER: S-EPMC2270789 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature