Identification of a contemporary human parechovirus type 1 by VIDISCA and characterisation of its full genome.

ABSTRACT:

Background

Enteritis is caused by a spectrum of viruses that is most likely not fully characterised. When testing stool samples by cell culture, virus isolates are sometimes obtained which cannot be typed by current methods. In this study we used VIDISCA, a virus identification method which has not yet been widely applied, on such an untyped virus isolate.

Results

We found a human parechovirus (HPeV) type 1 (strain designation: BNI-788st). Because genomes of contemporary HPeV1 were not available, we determined its complete genome sequence. We found that the novel strain was likely the result of recombination between structural protein genes of an ancestor of contemporary HPeV1 strains and nonstructural protein genes from an unknown ancestor, most closely related to HPeV3. In

SUBMITTER: de Souza Luna LK 

PROVIDER: S-EPMC2270820 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature