Protein kinase substrate identification on functional protein arrays.

ABSTRACT:

Background

Over the last decade, kinases have emerged as attractive therapeutic targets for a number of different diseases, and numerous high throughput screening efforts in the pharmaceutical community are directed towards discovery of compounds that regulate kinase function. The emerging utility of systems biology approaches has necessitated the development of multiplex tools suitable for proteomic-scale experiments to replace lower throughput technologies such as mass spectroscopy for the study of protein phosphorylation. Recently, a new approach for identifying substrates of protein kinases has applied the miniaturized format of functional protein arrays to characterize phosphorylation for thousands of candidate protein substrates in a single experiment. This method involves th

SUBMITTER: Meng L 

PROVIDER: S-EPMC2270825 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature