Quantitative multicolor compositional imaging resolves molecular domains in cell-matrix adhesions.

ABSTRACT:

Background

Cellular processes occur within dynamic and multi-molecular compartments whose characterization requires analysis at high spatio-temporal resolution. Notable examples for such complexes are cell-matrix adhesion sites, consisting of numerous cytoskeletal and signaling proteins. These adhesions are highly variable in their morphology, dynamics, and apparent function, yet their molecular diversity is poorly defined.

Methodology/principal findings

We present here a compositional imaging approach for the analysis and display of multi-component compositions. This methodology is based on microscopy-acquired multicolor data, multi-dimensional clustering of pixels according to their composition similarity and display of the cellular distribution of these composition cluste

SUBMITTER: Zamir E 

PROVIDER: S-EPMC2270910 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature