High vascular pressure-induced lung injury requires P450 epoxygenase-dependent activation of TRPV4.

ABSTRACT: High vascular pressure targets the lung septal network, causing acute lung injury. While calcium entry in septal endothelium has been implicated, the channel involved is not known. This study tested the hypothesis that the vanilloid transient receptor potential channel, TRPV4, is a critical participant in the permeability response to high vascular pressure. Isolated lungs from TRPV4(+/+) or TRPV4(-/-) mice were studied at baseline or during high pressure challenge. Permeability was assessed via the filtration coefficient. Endothelial calcium transients were assessed using epifluorescence microscopy of the lung subpleural network. Light microscopy and point counting were used to determine the alveolar fluid volume fraction, a measure of alveolar flooding. Baseline permeability, calcium inte

SUBMITTER: Jian MY 

PROVIDER: S-EPMC2274943 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature