Microarray analysis using disiloxyl 70mer oligonucleotides.

ABSTRACT: DNA microarray technology has evolved dramatically in recent years, and is now a common tool in researchers' portfolios. The scope of the technique has expanded from small-scale studies to extensive studies such as classification of disease states. Technical knowledge regarding solid phase microarrays has also increased, and the results acquired today are more reliable than those obtained just a few years ago. Nevertheless, there are various aspects of microarray analysis that could be improved. In this article we show that the proportions of full-length probes used significantly affects the results of global analyses of transcriptomes. In particular, measurements of transcripts in low abundance are more sensitive to truncated probes, which generally increase the degree of cross hybridizat

SUBMITTER: Bjorklund MG 

PROVIDER: S-EPMC2275082 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature