Actin binding domains direct actin-binding proteins to different cytoskeletal locations.

ABSTRACT:

Background

Filamin (FLN) and non-muscle alpha-actinin are members of a family of F-actin cross-linking proteins that utilize Calponin Homology domains (CH-domain) for actin binding. Although these two proteins have been extensively characterized, little is known about what regulates their binding to F-actin filaments in the cell.

Results

We have constructed fusion proteins consisting of green fluorescent protein (GFP) with either the entire cross-linking protein or its actin-binding domain (ABD) and examined the localization of these fluorescent proteins in living cells under a variety of conditions. The full-length fusion proteins, but not the ABD's complemented the defects of cells lacking both endogenous proteins indicating that they are functional. The localization patte

SUBMITTER: Washington RW 

PROVIDER: S-EPMC2275727 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature