Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen.

ABSTRACT:

Background

Pollens from different olive (Olea europaea L.) cultivars have been shown to differ significantly in their content in Ole e 1 and in their overall allergenicity. This allergen is, in addition, characterized by a high degree of polymorphism in its sequence. The purpose of this study is to evaluate the putative presence of divergences in Ole e 1 sequences from different olive cultivars.

Results

RNA from pollen individually collected from 10 olive cultivars was used to amplify Ole e 1 sequences by RT-PCR, and the sequences were analyzed by using different bioinformatics tools. Numerous nucleotide substitutions were detected throughout the sequences, many of which resulted in amino acid substitutions in the deduced protein sequences. In most cases variability within a

SUBMITTER: Hamman-Khalifa A 

PROVIDER: S-EPMC2275730 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature