Cross-competition in editing of chloroplast RNA transcripts in vitro implicates sharing of trans-factors between different C targets.

ABSTRACT: C-->U plant organellar RNA editing is required for the translation of evolutionarily conserved and functional proteins. 28 different C targets of RNA editing have been identified in maize chloroplasts, and hundreds of Cs are edited in mitochondria. Mutant analysis in Arabidopsis has indicated that absence of a single site-specific recognition protein can result in loss of editing of a single C target, raising the possibility that each C target requires a recognition protein. Here we show that transcripts encompassing two editing sites, ZMrpoB C467 and ZMrps14 C80, can compete editing activity from each other in vitro despite limited sequence similarity. The signal causing competition overlaps a 5'-cis element required for editing efficiency. A single five-nucleotide mutation spanning the r

SUBMITTER: Heller WP 

PROVIDER: S-EPMC2276328 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature