Defining the functional boundaries of the Gata2 locus by rescue with a linked bacterial artificial chromosome transgene.

ABSTRACT: Transcription factor GATA-2 is vital for both hematopoietic progenitor cell function and urogenital patterning. Transgenic mapping studies have shown that the hematopoietic and urogenital enhancers are located hundreds of kbp 5' and 3' to the Gata2 structural gene, and both are vital for embryonic development. Because the size of mammalian genes, including all of their associated regulatory elements, can exceed a megabase, transgenic complementation in mice has, in specific instances, proven to be a formidable hurdle. After incorporating the Gata2 structural gene as well as the distant hematopoietic and urogenital enhancers into a single, contiguous piece of DNA by fusing two bacterial artificial chromosomes (BACs) into one, we formally tested the hypothesis that the functional boundaries

SUBMITTER: Brandt W 

PROVIDER: S-EPMC2276365 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature