Microarray analysis of defined Mycobacterium tuberculosis populations using RNA amplification strategies.

ABSTRACT:

Background

The amplification of bacterial RNA is required if complex host-pathogen interactions are to be studied where the recovery of bacterial RNA is limited. Here, using a whole genome Mycobacterium tuberculosis microarray to measure cross-genome representation of amplified mRNA populations, we have investigated two approaches to RNA amplification using different priming strategies. The first using oligo-dT primers after polyadenylation of the bacterial RNA, the second using a set of mycobacterial amplification-directed primers both linked to T7 polymerase in vitro run off transcription.

Results

The reproducibility, sensitivity, and the representational bias introduced by these amplification systems were examined by contrasting expression profiles of the amplified produc

SUBMITTER: Waddell SJ 

PROVIDER: S-EPMC2276497 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature