Is Kir6.1 a subunit of mitoK(ATP)?

ABSTRACT: The subunit composition of the mitochondrial ATP-sensitive K(+)-channel (mitoK(ATP)) is unknown, though some suspect a role for the inward rectifier, Kir6.1, based largely on antibody studies of heart mitochondria. To ascertain the molecular identity of mitoK(ATP) we therefore sought to purify this putative mitochondrial Kir6.1, and conclusively identify the subunits by mass spectrometry. Immunoblots, conducted with two commercially available antibodies, revealed two distinct signals in isolated heart mitochondria, of 51 and 48kDa, respectively. Localization was confirmed by either immuno-gold electron microscopy or by immunofluorescence. Each putative Kir6.1 species was extracted, purified, and identified by LC-MS/MS. The 51kDa band was identified as NADH-dehydrogenase flavoprotein 1, whi

SUBMITTER: Foster DB 

PROVIDER: S-EPMC2276631 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature