Clinical improvement after treatment with VEGF(165) in patients with severe chronic lower limb ischaemia.

ABSTRACT: The present study focuses on the application of a therapeutic strategy in patients with chronic severe lower limb ischaemia using a plasmid vector encoding the vascular endothelial growth factor (phVEGF(165)). It has been shown that VEGF promotes neo-vascularization and blood vessel network formation and thus might have the ability to improve blood-flow at the level of the affected limbs. However, little information is available regarding the necessary level of expression of VEGF and its possible related adverse effects. We have subcloned VEGF ( 165 )isoform into pCMV-Script expression vector (Stratagene) under the control of the CMV promoter. Three patients with chronic ischaemia of the lower limb, considered as not suitable for surgical re-vascularization, received intramuscular injectio

SUBMITTER: Anghel A 

PROVIDER: S-EPMC2276892 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature