Cross-species EST alignments reveal novel and conserved alternative splicing events in legumes.

ABSTRACT:

Background

Although originally thought to be less frequent in plants than in animals, alternative splicing (AS) is now known to be widespread in plants. Here we report the characteristics of AS in legumes, one of the largest and most important plant families, based on EST alignments to the genome sequences of Medicago truncatula (Mt) and Lotus japonicus (Lj).

Results

Based on cognate EST alignments alone, the observed frequency of alternatively spliced genes is lower in Mt (approximately 10%, 1,107 genes) and Lj (approximately 3%, 92 genes) than in Arabidopsis and rice (both around 20%). However, AS frequencies are comparable in all four species if EST levels are normalized. Intron retention is the most common form of AS in all four plant species (~50%), with slightly lower

SUBMITTER: Wang BB 

PROVIDER: S-EPMC2277414 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature