Molecular epidemiology of Schistosoma mansoni: a robust, high-throughput method to assess multiple microsatellite markers from individual miracidia.

ABSTRACT: Schistosomiasis is one of the major unconquered infectious diseases afflicting people of developing countries, particularly in Africa. A deeper understanding of the epidemiology of schistosomes is complicated by the intravascular location of adult worms which makes them routinely unavailable for study. Their progeny, miracidia, which are hatched from eggs that are passed in feces, are available and can provide valuable insights about human infections, but they are small in size, hindering robust molecular analyses. Here we present a new high-throughput technique to assess the genotypes at 21 previously published microsatellite loci for individual miracidia of S. mansoni. The 21 loci can be amplified in four multiplexed PCR reactions; however, enough template is produced for approximately s

SUBMITTER: Steinauer ML 

PROVIDER: S-EPMC2278012 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature