Characterization of human mesenchymal stem cell secretome at early steps of adipocyte and osteoblast differentiation.

ABSTRACT:

Background

It is well established that adipose tissue plays a key role in energy storage and release but is also a secretory organ and a source of stem cells. Among different lineages, stem cells are able to differentiate into adipocytes and osteoblasts. As secreted proteins could regulate the balance between both lineages, we aimed at characterizing the secretome of human multipotent adipose-derived stem cell (hMADS) at an early step of commitment to adipocytes and osteoblasts.

Results

A proteomic approach, using mono-dimensional electrophoresis and tandem mass spectrometry, allowed us to identify a total of 73 proteins at day 0 and day 3 of adipocyte and osteoblast differentiation. Analysis of identified proteins showed that 52 % corresponded to classical secreted proteins

SUBMITTER: Chiellini C 

PROVIDER: S-EPMC2279142 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature