RRNA complementarity within mRNAs: a possible basis for mRNA-ribosome interactions and translational control.

ABSTRACT: Our recent demonstration that many eukaryotic mRNAs contain sequences complementary to rRNA led to the hypothesis that these sequences might mediate specific interactions between mRNAs and ribosomes and thereby affect translation. In the present experiments, the ability of complementary sequences to bind to rRNA was investigated by using photochemical cross-linking. RNA probes with perfect complementarity to 18S or 28S rRNA were shown to cross-link specifically to the corresponding rRNA within intact ribosomal subunits. Similar results were obtained by using probes based on natural mRNA sequences with varying degrees of complementarity to the 18S rRNA. RNase H cleavage localized four such probes to complementary regions of the 18S rRNA. The effects of complementarity on translation were as

SUBMITTER: Tranque P 

PROVIDER: S-EPMC22815 | biostudies-literature | 1998 Oct

REPOSITORIES: biostudies-literature